This study investigates the potential of Aneurinibacillus aneurinilyticus (A. aneurinilyticus), isolated from corn steep liquor, as a sustainable source of valuable bioproducts such us biosurfactants, Gramicidin S homologs, and polyhydroxyalkanoates (PHAs). Using phosphate-buffered saline (PBS), a biosurfactant extract was successfully extracted from A. aneurinilyticus cells, demonstrating its surface-active properties. In addition, ethanol was employed to extract Gramicidin S homologs from A. aneurinilycus cells, showcasing their potential to obtain new antibiotics against Gram-positive bacteria to address drug-resistant pathogens. Finally, vacuoles in A. aneurinilyticus were identified as potential reservoirs for PHAs, biopolyesters that can serve as sustainable alternatives to petroleum-based plastics.