Microbial Fuel Cell (MFC) technology is based on bioelectrochemical system, extract power from organic load of the wastes to produce bio-electricity. The present study have evaluated the effect of the different electrode materials in two sets of mediator-less H- type double chambered MFC operated at 30 ± 2 °C in a batch mode. In MFCGG, graphite rods (G) and in MFCCBG carbon brush (CB) and graphite rod (G) were used as anode and cathode electrode respectively. The both MFC were fed with distillery spent wash as a substrate with HRT of 21 days. The maximum COD removal of 61.07 % and 67.17 %; open circuit voltage (OCV) of 565 and 735 mV were achieved in MFCGG and MFCCBG respectively. The peak power densities of 3.19 W/m2 and 5.4 W/m2 were recorded in MFCGG and MFCCBG. These results suggest the efficacy of carbon brush anode in MFCCBG compared to graphite rod as an anode material in MFC for bioelectricity production.