Nowadays there is near consensus that cities have an important role to play in the pursuit of global climate protection and Sustainability. However, local strategies for sustainable development require a holistic and systemic approach, enabling multiscale involvement dynamics from a variety of stakeholders. Because Sustainability objectives are multifaceted, they often do not align with local governments' typical organizational structures, which compartmentalize expertise around core service functions. This paper intents to analyze the Governance Ecosystem Guimarães 2030 highlighting on the practical implementation of the quintuple helix model at local level. Available Information allowed characterizing Guimarães' Governance model as well as understanding how it evolved overtime. Projects were selected for each of the five-helix showcasing the continuous circulation of knowledge that is contributing for local sustainable development.